A 3-D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2-D environments (e.g. a Petri dish), a 3-D cell culture allows cells in vitro to grow in all directions, like how they would in vivo. We offer two Hydrogels - a biomimetic dextran-based hydrogel and a peptide-based hydrogel. Each hydrogel offers unique advantages for applications such as drug discovery and tissue engineering.
Antibodies are important tools in life science research. Besides the commonly used secondary antibodies, loading control antibodies, and epitope tag antibodies, we provide unique primary antibodies to meet diverse research needs such as HIV-1 related antibodies, bacterial toxins antibodies, viruses-related (e.g. HCV, HHV, VZV and Influenza) antibodies, and DNA 3R- related (i.e. replication, recombination and repair) antibodies have been very popular in these specialized areas.
Assay kits for metabolism, diabetes, obesity and apoptosis allows researchers to quickly narrow down results by detection target, species reactivity, application or conjugate. Our well-referenced Adiponectin, Leptin, Resistin, and esRAGE, Insulin, GDPH, Creatinine, AVP ELISA kits provides a broad linear detection range and picograms per milliliter sensitivity. The highly specific antibodies provide consistent and reliable colorimetric readings. In addition, our caspases assay kits, cathepsins assay kits and FLISP kits help advance your apoptosis studies.
Microgravity provides a special environment where cells grow without sedimentation and convection. Several studies have shown that the formation of 3-D aggregates after the cells were cultured under microgravity conditions. The 3-D multicellular spheroids or tissues represent more physiologically relevant in vivo situations needed for biomedical research and drug development.
The Histofine IHC (Immunohistochemistry) detection kits for human, mouse, rabbit, and goat primary antibodies use just the antigen-recognizing Fab fragment (rather than a whole antibody) to detect a primary antibody. This feature minimizes non-specific binding, thereby increasing the signal-to-noise ratio and generating cleaner results.
In the life science industry, live cells or tissues are usually frozen with cryoprotectants and transported with dry ice if they need to be moved over long distances. However, these conventional procedures are associated with problems such as the loss of cells while freezing or thawing. With the iP-TEC® Live Transport Device, cells can be transported without freezing, and they would be ready to use once they arrive at the intended destination.
PluriBead is an innovative separation technology for the isolation of cells, proteins, viruses, bacteria and a variety of other targets from whole blood, cell culture, cord blood and other heterogeneous biological fluids. The tools are versatile in application and form the basis of research in cancer, rheumatic illnesses, arteriosclerosis and allergies.
Red/ET Recombineering offers unprecedented freedom in gene cloning. It gives you the powerful tools to clone, subclone, and modify DNA at any chosen position. These Red/ET Kits, Selection Cassettes, and Recombinase Plasmids permit precise engineering of DNA molecules of any size, including very large ones such as BACs or the E. coli chromosomes.
The ShellPa Mechanical Cell Strain System and cell culture chambers allow researchers to explore the impact of mechanical cell strain on cells in culture. Mechanical stress applied to the cells better simulates a natural dynamic physiological environment. ShellPa is an instrument for cell culturing that has been developed to replicate a dynamic environment inside the human body.
Stable cell lines are one of the most used tools in drug discovery, toxicity testing, and basic research. Long-term stable expression of a gene of interest is usually achieved by transfection, or viral transduction of a vector containing the expression cassette of the gene of interest, together with a selection marker (i.e. antibiotics or fluorescent proteins). In contrast to transient expression, stable cell lines offer reproducible results for assay studies in drug discovery and development. We provide stable cell lines with constitutive expression of a wide variety of genes, including GPCRs, ion channels, proteases and kinases as well as various other enzymes, transmembrane proteins and adhesion molecules. These cell lines are designed for High Content Screening (HCS) methodologies.
Nucleic acid isolation is one of the most basic techniques performed in every molecular biology lab, yet it is vitally important for the success of all downstream applications. At the core of STRATEC products is the unique non-chaotropic chemistry. Unlike traditional buffers that contain denaturing (chaotropic) ions such as guanidinium, STRATEC uses non-chaotropic ions that provides major advantages such as faster protocol, high DNA yield and intact DNA.
The TumorImager is a next generation scanner for subcutaneous tumor measurements on small lab animals. It uses a unique structured light imaging system to capture both a 3D surface profile and a color image. The patented algorithms can also isolate a tumor in the recorded image and calculate the volume or area. When interfaced to the TumorManager software, this leads to a simple but robust, data-verified measurement system.
Cas9 is provided as a purified protein, which allows for immediate activity without transcription or translation and has been shown to reduce off-target effects. The Cas9 protein has an N-terminal and a C-terminal nuclear localization signal, which allows for more efficient crossing of the nuclear membrane in order to generate double-stranded breaks in DNA with high efficiency and specificity. Cas9 forms an active ribonucleoprotein complex with the sgRNA. Cas9 protein requires an sgRNA towards the target sequence for gene editing applications.
Presented in a simple box, our CRISPR Complete kits contain everything you need to perform your CRISPR experiments. These kits are tailor-made for your project by our team of genetic engineers, who have extensive experience editing cells and animal models. CRISPR kits are available for almost any project type, including but not limited to knockouts, knock-ins, TUNR knockdowns, point mutations, gene replacements, humanizations, and tagging.
We provide a large collection of CRISPR-Cas9 Knockout Cell Lines generated by gene editing in popular cell lines such as HEK293T, HeLa and A549. They are 100% clonal cell lines - and no pools. Both Cell Lines and Lysates are available.
Single guide RNAs are typically used when generating gene knockouts with the CRISPR-Cas9 system. This introduces double-strand breaks, which the cell attempts to repair with NHEJ. NHEJ can be imperfect, leading to small insertions and deletions, causing frameshift mutations and the introduction of early stop codons. It is the introduction of these early stop codons that leads to gene knockout. Due to reliance on the generation of early stop codons, single guide RNA systems are not effective when knocking out noncoding RNAs, including miRNA. To address this challenge, a dual-gRNA construct system is used to target flanking genomic regions of the miRNA gene, leading to complete excision of the miRNA gene.
The TUNR Flexible Gene Editing System allows you to precisely control the expression of your gene of interest. Our kits are custom-designed for your project and include everything you need to tune gene expression in your favorite cell line. The TUNR Flexible Gene Editing System leverages the CRISPR-Cas9 system for targeted integration of a polyA track at a precise location within the targeted gene.
Drug developers and researchers need crucial information that is often hidden deep inside rare cells that circulate in our body. These cells provide a plethora genomic and cellular information, from gene mutations to protein expression profiles. The MiCareo system captures, stains and acquires fluorescent multiplexed high dimensional images of rare cells from whole blood.